How to Perform a Siphoning Test for AKTA Chromatography Systems
Siphoning occurs in a chromatography system due to the tubing flow path's configuration. It happens when the outlet point is positioned lower than the reservoir, creating a vertical distance that drives flow under gravitational pressure. In chromatography systems, another scenario can occur where one pump is active while the other is idle. This can lead to a phenomenon where the active pump creates a vacuum or low-pressure area that causes fluid to be drawn from the inlet line of the idle pump.